Table 1.

DSC data for inhibitor binding to PR, PR_D25N and PR_D29N

Inhibitor	Tm (°C)			ΔTm (°C)			KL at Tm (μM)a			KL at 25 °C (nM)
	PR	PRD25N	PRD29N	PR	PRD25N	PRD29N	PR	PRD25N	PRD29N	PR (lit.)	PR (calc.)a
none	65.7	58.4	59.0	-	-	-	-	-	-	-	-
DRV	88.1	61.5	72.7	22.4	3.1	13.7	0.018	16.6	1.34	0.005b; 0.010c	0.014
ATV	86.5	61.4	71.7	20.8	3.0	12.7	0.036	17.4	1.80	0.035 c	0.186
SQV	85.0	62.6	72.3	19.3	4.2	13.3	0.066	10.0	1.51	0.28d,e	8.14
RTV	81.3	61.1	66.1	15.6	2.7	7.1	0.27	20.5	9.3	0.10d,e	9.72
APV	80.0	58.7	67.5	14.3	0.3	8.5	0.44	274	6.2	0.2d,e	3.46
DMP323	81.0	67.3	69.2	14.7	8.9	10.2	0.30	1.6	3.7	3.8 f	-
RPB	74.4	64.6	59.2	8.7	6.2	0.2	2.9	4.4	1178	50 g	-
Ac-PEP	72.2	58.8	58.3	6.5	0.4	-	6.1	202	-	440 e	2956

^aLigand dissociation constants calculated by the method of Brandts and Lin²⁷ with ΔH = 43.3, 53.1 and 26.4 kcal/mol (this work) for PR, PR_D25N and PR_D29N, respectively. ΔC_P = 3 kcal/mol·degree as measured²⁵,²⁶ for PR was used in calculations for all three constructs. K_L values for PR at T_m (this work) and at 25 °C are italicized for ease of comparison.

Cited from Refs.

^b12

^c39

^d26

^e40

^f38

^g17