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. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Apr 19;358(1):111–116. doi: 10.1016/j.bbrc.2007.04.064

Figure 3. BMP synthesis is reduced in detergent resistant microdomains isolated from JNCL patient derived fibroblasts.

Figure 3

(A) Lipid standards. (B) Control and CLN3-deficient human fibroblast cell lines were incubated with [3H]-palmitate for 18 hours. Equivalent number of cells were harvested and DRMs were isolated. DRM lipids were extracted, separated by 2D-HPTLC, and visualized by autoradiography. BMP is indicated by the arrow. Bands were excised from the TLC plates and [3H]-palmitate incorporation was measured for each of the lipid species. Results are representative of at least three independent experiments in three independent patient cell lines.