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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: J Mol Biol. 2009 Jun 21;391(2):275–281. doi: 10.1016/j.jmb.2009.06.037

Figure 1.

Figure 1

Sequence and structure of tmRNA. A) Secondary structure of E. coli tmRNA, including the tRNA-like domain (TLD), four pseudoknots (PK1-4), and the resume and stop codons boxed at either end of the tag template sequence.32 B) Fragment of the tmRNA sequence including the first five codons in the tag template as well as five nucleotides of the upstream sequence known to play a role in frame selection. The resume codon is underlined. The His 0 template encodes ANDH6D in the natural frame. Addition of a G before G90 leads to the synthesis of this tag only when the +1 frame is read in the His +1 reporter. Likewise, deletion of C98 leads to synthesis of RKRH6D only upon translation in the −1 frame in the His −1 reporter.