Figure 2.

Co-ligation of Dectin-1 and TLR2 enhances production of IL-6 and IL-23 but down-regulates IL-12. (A) Production of IL-6 by C57BL/6 BMDC stimulated with the indicated concentrations of Pam₃CSK₄ with or without 10 μg/mL β-glucan. (B) IL-6 production induced by 10 μg/mL β-glucan or 1 μg/mL LPS in 129/Sv WT and Dectin-1^−/− BMDC. (C) Collaborative induction of IL-23 by C57BL/6 BMDC stimulated with the indicated concentrations of Pam₃CSK₄ with or without 10 μg/mL β-glucan. (D) Co-stimulation of C57BL/6 BMDC with the indicated concentrations of Pam₃CSK₄ and 10 μg/mL β-glucan down-regulates production of IL-12p70 relative to levels after Pam₃CSK₄ stimulation alone. Data shown are mean±SD and are representative of at least two independent experiments.