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. 2009 May;39(5):1379–1386. doi: 10.1002/eji.200838543

Figure 4.

Figure 4

Down-regulation of IL-12 is dependent on signaling through Dectin-1. (A) Phosphorylation of Syk and Akt after stimulation of Balb/C BMDC with 10 μg/mL soluble β-glucan and 1 μg/mL Pam3CSK4, as indicated. Production of IL-10 (B) and IL-12p70 (C) from Balb/C Syk−/− or WT thioglycollate-elicited macrophages pretreated with vehicle, 20 μM U0126 or 20 μM Ly294002, and stimulated with 10 μg/mL β-glucan and 10 ng/mL Pam3CSK4, as indicated. (D) Production of IL-12p70 from Balb/C WT or 129Sv Dectin-1−/− BMDC pretreated with vehicle, 20 μM U0126 or 20 μM Ly294002, and stimulated with 10 μg/mL β-glucan and 100 ng/mL Pam3CSK4, as indicated. (E) Production of IL-12p70 from Balb/C WT or IL-10−/− BMDC stimulated with 10 μg/mL β-glucan and 100 ng/mL Pam3CSK4, as indicated. Data shown are mean±SD and are representative of two independent experiments.