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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):207–215. doi: 10.4049/jimmunol.182.1.207

Figure 4. Proliferation and Ab responses in b-cflip−/− B cells.

Figure 4

(A). Splenic and lymph node B cells were stimulated with the indicated agonists. Proliferation was determined by the incorporation of radioactivity of [3H] thymidine (cpm, counts per minute). (B). B cell division kinetics were analyzed by CFSE dilution assays. At 48 h after stimulation, CFSE-labeled B cells were stained with 7AAD and analyzed by flow cytometry. The percentages of cell death are indicated by the 7AAD+ populations. (C) Cell death was determined at various times following LPS stimulation and death curves generated. (D) TLR3 expression in B cells was determined by western blotting. (E) TLR4 expression in B cells was analyzed by flow cytometry.