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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: J Proteome Res. 2009 Jun;8(6):2740–2751. doi: 10.1021/pr801040h

Figure 2. NB-DGJ completely abolishes the NKT cell development.

Figure 2

Fetal thymus organ culture was performed as previously described (22). Thymuses from fetal mice (Day 14 after pregnancy) were cultured in 6 well transwell plates (Corning, NY) in EHAA/RPMI medium with 10% Fetal Calf Serum. NB-DGJ (10 μg/ml and 100 μg/ml) was added at the beginning of culture. The thymuses were cultured to allow the development of NKT cells. After 14 days, the thymocytes were harvested and analyzed by flow cytometry after staining by αGalCer/CD1d tetramer and anti-mouse CD44 monoclonal antibody (Pharmingen, CA). To exclude the possibility of toxicity to thymocytes, the development of conventional CD4 and CD8 T cells were used as controls. No reduction of CD4 or CD8 numbers was found in either percentage or total cell numbers.