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. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: Cancer Cell. 2008 Aug 12;14(2):180–192. doi: 10.1016/j.ccr.2008.06.014

Figure 4. Growth inhibition screen of p110α mutant libraries in S. cerevisiae.

Figure 4

A: YRP1-pURA3-2μ-GAL1-p110αH1047R-CAAX mutant library pinned onto SD −URA +Galactose and grown for 5 days at 30°C, and the distribution of colony sizes for that plate, relative to the same array plated on SD −URA +Glucose and grown for 3 days at 30°C (not shown).

B: Representative image of pooled active clones from YRP1-pURA3-2μ-GAL1-p110αH1047R-CAAX mutant libraries, pinned onto SD −URA +Galactose with either DMSO control or 5 μM PI-103 and grown for 5 days at 30°C, and the corresponding distributions of colony sizes, relative to the same array plated on SD −URA +Glucose and grown for 3 days at 30°C (not shown).

C: Ten-fold serial dilutions of YRP1-pURA3-2μ-GAL1-p110αH1047R-CAAX strains with the indicated p110α mutations spotted onto SD −URA +Galactose media containing the indicated PI3K inhibitors and grown for 5 days at 30°C.