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. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: Cancer Cell. 2008 Aug 12;14(2):180–192. doi: 10.1016/j.ccr.2008.06.014

Figure 6. Yeast screen hits I800L, I800M, and L814C confer EGF-independent growth to MCF10A cells, and maintain altered inhibitor sensitivities in vivo.

Figure 6

A: MCF10A cell lines expressing the indicated p110α mutants were cultured in growth media lacking EGF, and monitored for growth by Alamar Blue assay. Data are represented as mean ± SEM.

B and C: Growth of the I800L, I800M, and L814C MCF10A cell lines in media lacking EGF was monitored as in Fig. 6A in the presence of the indicated PI3K inhibitors. Data are represented as mean ± SEM.