Skip to main content
. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: Bioorg Med Chem. 2007 Nov 26;16(4):2114–2130. doi: 10.1016/j.bmc.2007.10.081

Figure 7.

Figure 7

Alignment of esterases examined in this study using BioEdit v. 7.0.9 (ClustalW alignment61 using a BLOSUM62 matrix displayed with an 80% threshold). hCE-1=human carboxylesterase 1 (NM_001025194), hCE-2=human carboxylesterase 2 (NM_003869), pCE=porcine esterase (NM_214246), rCE=rabbit esterase (AF036930), MsJHE= juvenile hormone esterase from Manduca sextra (AF327882). The catalytic triad is marked with a black box (Ser229, Glu364 and His481; His484 for MsJHE) and the glycines involved in oxyanion hole formation are shown in a blue box (Gly 148 and Gly 149). The putative conserved second serine involved in the catalytic mechanism is also shown in a black box (Ser255).62 The residue involved in pi-stacking interactions in MsJHE is marked with a red box (Phe262) and the ER retention sequence is shown in a green box (HXEL).