Table 2. Changes in expression of G-proteins after 8-h stimulation by 10 nM DHT.
Maximal fold change upregulation
|
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OVCAR3
|
OSEC2 | ||||
Gene | Accession number | Brief description of known function | Affymetrix | RT-PCR | RT-PCR |
GNA13 | NM_006572 | α-Subunit of heterotrimeric G-protein 13 | 2.2 | 5.5 | 146.1 |
ELKS | AB029004 | RAB6-interacting protein 2. Regulator of NF-κB signalling | 2.9 | 2 | 1.4 |
GSTP1 | BE544748 | G to S-phase transition protein 1 | 2.5 | 2 | 7.8 |
RERG | AW294092 | Monomeric G-protein | 2.9 | 3 | 5.2 |
Rab25 | NM_020387 | Monomeric G-protein. Involved in vesicle trafficking | 1.05 | 1.42 | ND |
Rab45 | NM_152573 | Monomeric G-protein. Involved in vesicle trafficking | 2.5 | 2.5 | 8.6 |
Rab35 | NM_006861 | Monomeric G-protein. Involved in vesicle trafficking | 5.4 | 13 | 13.1 |
DHT=dihydrotestosterone; NF-κB=nuclear factor κB; RT-PCR=reverse transcriptase PCR.