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Extrusion of the second polar body fails in rec-8 mutants. (A) Polar bodies in DAPI-stained embryos. Arrows indicate the two polar bodies of wild-type and spo-11 mutants and the single polar body of rec-8 and spo-11 rec-8 mutants. (B–D) A single polar body is extruded in most rec-8, spo-11 rec-8 dpy-4, htp-1 htp-2 (see also Martinez-Perez et al. 2008), and htp-3 mutant embryos. In contrast, two polar bodies are present in most wild-type, spo-11, him-3, and htp-1 mutant embryos.
