Figure 1.

Coiled coils and the yeast genome. (A) A Schematic representation of a two-stranded coiled coil showing its characteristic heptad repeat (abcdefg)_n. Residues at the a and d positions (in red) are predominantly hydrophobic, whereas those at the e and g positions (blue) are frequently charged or polar (14). For simplicity, the superhelical twist of the two helices is not depicted. (B) The coiled-coil probabilities of translated yeast ORFs scoring ≥0.2 (dimer + trimer). The majority of sequences used in the pilot screen (red squares) have high dimeric and low trimeric probabilities. Sequences used in the directed screen (blue diamonds) show more variation. Sequence scores (3) for the remaining translated ORFs are shown as black dots. (C) Properties of translated yeast ORFs that are predicted to contain the two-stranded coiled-coil interaction motif. multicoil identifies 490 sequences in the translated yeast genome with ≥0.2 probability of forming a two-stranded coiled coil. Data are derived from YPD, Ver. 9.46A (40).