Figure 3.

Coiled-coil interactions identified in this study. pGAD (activation domain) constructs are listed (Top, discontiguous numbering), with pGBDU (DNA-binding domain) constructs (Right, contiguous numbering) (see also Table 1). The common names of the full-length proteins from which the constructs are derived are also listed. Because of space constraints, pGAD constructs making no interactions have not been included. Homotypic interactions are shown as open boxes, heterotypic interactions as closed boxes (see also Table 2). Although many of these interactions are likely to form two-stranded coiled coils, some may form higher-order oligomers. Additionally, we cannot distinguish parallel from antiparallel coiled coils. Six of the interactions shown here have been previously documented (Smc1p/Smc2p; Smc1p/Smc3p; Met4p/Met28p; Met4p/Met4p; Kel1p/Kel1p; and Spc42p/Spc42p) (40).