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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: J Am Chem Soc. 2009 Jul 22;131(28):9695–9703. doi: 10.1021/ja9006707

Figure 1. Self-assembled ssDNA-p/MHC tetramer arrays for multiplexed sorting of antigen-specific cells.

Figure 1

ssDNA-tagged p/MHC tetramers are produced by coupling ssDNA site-specifically to SAC prior to exposure to molar excess of biotinylated p/MHC monomers. p/MHC tetramer arrays are formed by pooling ssDNA-p/MHC tetramers of select specificity and hybridization to a complementary printed ssDNA microarray. T cells expressing the cognate TCR are detected by binding to the surface confined tetramer.