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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: J Am Chem Soc. 2009 Jul 22;131(28):9695–9703. doi: 10.1021/ja9006707

Figure 5. Programmed release of sorted T cells by endonuclease cleavage.

Figure 5

(A) DNA microarrays were printed with orthogonal sequences containing EcoRI and BamHI restriction sites. (B) Fluorescent images of Jurkatα-MART-1 (red) and Jurkatα-Tyro (green) cells captured on p/MHC array (i) and after treatment with BamHI (ii) or EcoRI (iii). Only cells localized to DNA spots containing the target restriction sequence were released. A second round of enzymatic treatment released the remaining bound cells (iv, image representative of BamHI to EcoRI cleavage).