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. 2009 Jul 16;6:106. doi: 10.1186/1743-422X-6-106

Table 2.

Primers.

Forward Primer Reverse Primer
Envelope 1st
(1042–1857)		 5'-GAAGGCGATAGTTGTGTGACCA-3'
(1042–1063)		 5'-TGTTCCCTTCAGCTGCAACTT-3'
(1834–1854)
Envelope 2nd
(1632–2459)		 5'-CCTTGGAGCAGTGCTGGAAGTA-3'
(1636–1657)		 5'-TTCACGGAGAGGAAGAGCAGAA-3'
(2438–2459)
NS3 1st
(5085–5908)		 5'-CGGCTCATACATAAGCGCGAT-3'
(5085–5105)		 5'-TTGGTTTCACACTCTTCCGGC-3'
(5888–5908)
NS3 2nd
(5514–6318)		 5'-TTCCACAAAGGTCGAGCTAGG-3'
(5514–5534)		 5'-CCTAGGACCATCAAAGCACCA-3'
(6298–6318)
NS3 3rd
(5950–6726)		 5'-CCATCTGCAGTGACAGCAGCTA-3'
(5950–5971)		 5'-TTCGTTCCTGGAACTTCAGCC-3'
(6756–6776)

Primer sequences used to sequence the envelope and NS3 regions of the samples.

*NT positions refer to amplicon location with respect to "WNV RNA, Complete Genome" GenBank accession number M12294