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. Author manuscript; available in PMC: 2010 Aug 20.
Published in final edited form as: J Proteomics. 2009 Jan 20;72(6):907–917. doi: 10.1016/j.jprot.2009.01.007

Figure 1. SDS-PAGE separation of different prostatic disease EPS urine pools.

Figure 1

Individual EPS urine sample pools (n=6) representative of BPH, low risk prostate cancer (PCa), high risk PCa and metastatic PCa conditions were generated by 10X concentration through a 5000 MW Amicon cut-off filter (Millipore). Protein loading was normalized to 20 ug total protein per pool, and separated on a 12% Bis-Tris NuPAGE SDS-gel (Invitrogen) visualized by silver staining (Invitrogen). The indicated protein band at 17 kDa was identified by LC-MS/MS as β-2-microglobulin (β-2-MG; Mascot score of 1264), essentially as described in the legend to Table 1.