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Effects of wild-type and mutant λcI on transcription in the presence of α-σ70 chimeras. SF1 cells harboring the indicated plasmids were assayed for β-galactosidase activity. pACYC-derived plasmids encoded either λcI (pACλcI) or λcI(D38N) [pACλcI(D38N)]; pBR322-derived plasmids encoded either the α-σ70 chimera (pBRα-σ70) or the α-σ70 (R596H) chimera [pBRα-σ70 (R596H)].
