Figure 3.

Dose-responsive activation of gene expression by rapamycin and a rapamycin analog in L4-S3H-ZF1-S-infected HT1080 cells. SEAP activity secreted into the growth medium was measured after a 24-h incubation of cells with the indicated concentration of rapamycin or AP22565. SEAP gene expression was assayed as described in the legend to Fig. 1B, except that the growth medium was diluted 10-fold before incubation with SEAP reaction buffer. Assays were performed in triplicate, and mean values (in arbitrary units, AU) ± SD were plotted.