Figure 2.
False peptide identification due to misinterpretation of charge states. (A) Assignment of MS/MS spectrum with a triply charged peptide, ASGVPDKFSGSGSGTDFTLK, identified by the Mascot algorithm with a Mascot score of 39. (B) Assignment of the same MS/MS spectrum with a doubly charged peptide, FSGSGSGTDFTLK by the Mascot algorithm with Mascot score of 71.