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False peptide identification caused by misassignment of modification site. (A) Assignment of an MS/MS spectrum with a doubly charged peptide, YPIMeEHGIVTNWDDMEK, identified by Mascot with a score of 54. (B) Assignment of the same MS/MS spectrum with a doubly charged peptide, YPIEMeHGIVTNWDDMEK, identified by manual inspection.
