Table 1. Summary of Huh7 Cell Line Characteristics^a.

Cell Line	CPEb	Average HCV Copies/µg RNAd	Average HCV Titers (FFU/ml)e	dsRNA Signalingf	JFH-1 Infectivityg
Huh7-1	+ (8)c	3.04×107	4.67×104	++++	++++
Huh7-2	++ (7)	2.68×107	1.23×105	+++	++++
Huh7-3	++++ (5)	2.94×107	1.37×105	++++	++++
Huh7-4	+++ (6)	2.53×107	2.00×104	++++	++++
Huh7-6	+ (8)	2.82×103	3.67×101	+++	+
Huh7-7	++++ (5)	2.35×107	3.33×104	++++	++++
Huh7-8	++ (7)	1.39×107	1.00×104	+++	++++
Huh7-9	++++ (5)	4.08×107	1.43×105	++	++++
Huh7-RRB	+ (8)	1.68×107	7.11×104	n/d	++++
Huh7.5.1	++++ (5)	2.44×107	1.87×105	n/d	++++

^aRefer to Materials and Methods for definition of assays.

^bCPE determined by microscopic examination. Changes in cell morphology, accumulation of floating cells and cessation of cell growth were scored based on the percentage of cells in the infected culture exhibiting these characteristics: +++, high (>80%); ++, medium (40–80%); + low (<40%); and undetectable.

^cDay p.i. CPE was first evident.

^dAverage intracellular HCV RNA (copies/µg RNA) for days 6–8 p.i. (Figure 5A).

^eAverage infectivity titers (FFU/ml) for days 6–8 p.i. using respective Huh-7 cell line for assay (Figure 5B).

^fAverage ISG expression following poly(IC) transfection: ++++, >10-fold; +++, 3–10-fold; ++, <3-fold. (Figure 7B).

^gHCV spread determined by indirect E2 immunofluorescence staining on day 7 p.i.: ++++, >90%; +++, 60–89; ++, 30–59%; +, <30% E2-positive cells (Figure S1).

n/d = not determined.