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. 2009 Jul 1;29(26):8530–8538. doi: 10.1523/JNEUROSCI.0468-09.2009

Figure 3.

Figure 3.

Whole-cell patch-clamp recordings from Purkinje cells in different lobules and subregions reveal local DISC enrichment. A, A diagram of the cerebellar sagittal slice with recording electrodes. Recordings were made from Purkinje cells in different cerebellar regions: lobules II and III (gyrus and bank regions), lobules V and VI (bank regions), and lobules IX and X (gyrus and bank regions). The red shading indicated those areas in which DISC recordings were obtained. B, Representative single-current traces from different lobules are shown, together with high-pass-filtered traces to reveal the noise envelope. The noise envelope allows for the dissociation of DISC from the earlier Cl current. Calibration: 200 pA, 2 s for current traces. A range of −30 to 30 pA was used to depict the high-pass-filtered noise envelope. C, Population analysis of DISC in various lobules. The left panel shows the proportion of cells with DISC >100 pA. The right panel shows the current density of DISC, normalized to cell capacitance and calculated only for those currents >100 pA.