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. Author manuscript; available in PMC: 2009 Aug 4.
Published in final edited form as: J Neurochem. 2009 Feb 13;109(3):733–743. doi: 10.1111/j.1471-4159.2009.05990.x

Fig. 1. BMP6/7 and a BMP-neurotrophin combination increase nuclear and cytoplasmic P-Smad levels.

Fig. 1

Septal cells at 5 days in vitro were treated for 1 h with BMP6/7 alone or in combination with BDNF+NGF, then stained with an anti-P-Smad 1/5/8 antibody and counterstained with DAPI. Average values of nuclear and cytoplasmic fluorescence were measured as detailed in Fig. 1 of Supporting Information. A) Log scale scatter plot of P-Smad fluorescence in the nucleus (y axis) and cytoplasm (x axis). Each point represents one neuron. Points situated above the 45° identity line represent cells in which nuclear fluorescence exceeded cytoplasmic fluorescence. B) Ratio of nuclear to cytoplasmic P-Smad fluorescence, a measure of nuclear translocation. * indicates significant difference from control or between the indicated trophic factor groups, assessed with one-way ANOVA followed by Newman-Keuls test (p<0.05, n≥22 cells per group, from two different experiments).