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. Author manuscript; available in PMC: 2010 Jan 10.
Published in final edited form as: Cell. 2009 Jul 10;138(1):63–77. doi: 10.1016/j.cell.2009.06.030

Figure 5. SLX4-dependent cleavage of HJs maps to the C-terminal MUS81-EME1 and SLX1 binding domain (SBD).

Figure 5

(A) Schematic of SLX4 deletion fragments and relevant interactions.

(B) The indicated SLX4 complexes (from 1.5mg of cell extract) were incubated with radiolabeled substrates (30 min) labeled on strand 1 prior to native gel electrophoresis and autoradiography.

(C) Purified endonucleases produced in bacteria.

(D, E) SLX4SBD stimulates static and migrating HJ cleavage by SLX1. The indicated recombinant GST-SLX1-His6-SLX4SBD complexes (1, 3, or 5 ng) were incubated with XO-BE or X26 HJs at 37°C for 30 min and reaction products analyzed on native gels (panel D) or by phosphoimaging (panel E). SLX1CD: SLX1E82A.