(A) Schematic of SLX4 deletion fragments and relevant interactions.
(B) The indicated SLX4 complexes (from 1.5mg of cell extract) were incubated with radiolabeled substrates (30 min) labeled on strand 1 prior to native gel electrophoresis and autoradiography.
(C) Purified endonucleases produced in bacteria.
(D, E) SLX4SBD stimulates static and migrating HJ cleavage by SLX1. The indicated recombinant GST-SLX1-His6-SLX4SBD complexes (1, 3, or 5 ng) were incubated with XO-BE or X26 HJs at 37°C for 30 min and reaction products analyzed on native gels (panel D) or by phosphoimaging (panel E). SLX1CD: SLX1E82A.