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Effect of isoflavones on the levels of IL-4 mRNA and protein from PBMCs. The IL-4 mRNA was determined by RT-PCR and the IL-4 protein by ELISA assay.
Notes: *P < 0.05; **P < 0.01 as compared with the PHA + V control level.
Abbreviations: IL, interleukin; mRNA, messenger ribonucleic acid; PBMCs, peripheral blood mononuclear cells; PHA, phytohemagglutinin; RT, reverse transcriptase-polymerase chain reaction.
