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. Author manuscript; available in PMC: 2009 Aug 4.
Published in final edited form as: Nat Protoc. 2009;4(3):341–355. doi: 10.1038/nprot.2008.250

Figure 4.

Figure 4

Agarose gel analyses showing the synthetic Flag/MaSp 2 silk DNA multimers at different doubling stages. The sequential recombinant plasmids containing the different silk-like insert fragments43 were subjected to restriction digestion with XmaI and BspEI to release the silk insert. The restriction digestion products were separated on (a) Nusieve/agarose 3:1 and (b,c) 0.8–1% agarose gels. After staining with ethidium bromide, the DNA fragments were visualized using UV light. In a–c, Mk: molecular marker; Mk 1:1 kbp DNA Ladder; Mk 2: Lambda DNA-HindIII; 2×−16×: repetitive synthetic silk sequences after sequential insert doubling (i.e., in a, 4× is twice the size of 2×). The size of the silk inserts are 236, 472, 944 and 1,888 bp for 2×, 4×, 8× and 16×, respectively. The black arrows show the linearized pBluescript plasmid and the white arrows show the silk inserts. The molecular weights in kbp are indicated.