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. Author manuscript; available in PMC: 2009 Aug 4.
Published in final edited form as: Nat Protoc. 2009;4(3):341–355. doi: 10.1038/nprot.2008.250

Figure 5.

Figure 5

Synthetic silk fiber formation by extrusion. The pure lyophilized silk recombinant protein was solubilized in 100% HFIP. The silk spinning dope was loaded in the spinneret constituted of a glass syringe attached to PEEK tubing. Manual extrusion of the dope into a 90% isopropanol coagulation bath (a) generated a uniform silk fiber (b). The photograph in b was taken using a Nikon Coolpix 950 digital camera. The white bar in b represents 1 cm.