Skip to main content
. Author manuscript; available in PMC: 2009 Aug 4.
Published in final edited form as: Nat Protoc. 2009;4(3):341–355. doi: 10.1038/nprot.2008.250
Component Amount (µl) Final
10× NEB buffer 2 5 1×
BSA (1 mg ml−1) 5 100 µg ml−1
pBluescript/monomer DNA (1 µg µl−1) 2 2 µg
ScaI (10,000 U ml−1) 0.4 4 U
XmaI (10,000 U ml−1) 0.4 4 U
Nuclease-free water 37.2