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. Author manuscript; available in PMC: 2009 Aug 4.
Published in final edited form as: Nat Protoc. 2009;4(3):341–355. doi: 10.1038/nprot.2008.250
Component Amount (µl) Final
10× NEB buffer 3 5 1×
pBluescript/monomer DNA (1 µg µl−1) 2 2 µg
ScaI (10,000 U ml−1) 0.4 4 U
BspEI (10,000 U ml−1) 0.4 4 U
Nuclease-free water 42.2