Component | Amount (µl) | Final |
---|---|---|
10× NEB buffer 3 | 5 | 1× |
pBluescript/monomer DNA (1 µg µl−1) | 2 | 2 µg |
ScaI (10,000 U ml−1) | 0.4 | 4 U |
BspEI (10,000 U ml−1) | 0.4 | 4 U |
Nuclease-free water | 42.2 |
Component | Amount (µl) | Final |
---|---|---|
10× NEB buffer 3 | 5 | 1× |
pBluescript/monomer DNA (1 µg µl−1) | 2 | 2 µg |
ScaI (10,000 U ml−1) | 0.4 | 4 U |
BspEI (10,000 U ml−1) | 0.4 | 4 U |
Nuclease-free water | 42.2 |