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. Author manuscript; available in PMC: 2009 Aug 4.
Published in final edited form as: Nat Protoc. 2009;4(3):341–355. doi: 10.1038/nprot.2008.250
Component Amount Final
5× ligase reaction buffer 4 µl 1×
NdeI–BamHI pET-19b vector ends 3–30 fmol 3–30 fmol
NdeI–BamHI multimer insert ends 9–90 fmol 9–90 fmol
T4 DNA ligase (1 U µl−1) 1 µl 1 U
Nuclease-free water Up to 20 µl