Component | Amount | Final |
---|---|---|
5× ligase reaction buffer | 4 µl | 1× |
NdeI–BamHI pET-19b vector ends | 3–30 fmol | 3–30 fmol |
NdeI–BamHI multimer insert ends | 9–90 fmol | 9–90 fmol |
T4 DNA ligase (1 U µl−1) | 1 µl | 1 U |
Nuclease-free water | Up to 20 µl |
Component | Amount | Final |
---|---|---|
5× ligase reaction buffer | 4 µl | 1× |
NdeI–BamHI pET-19b vector ends | 3–30 fmol | 3–30 fmol |
NdeI–BamHI multimer insert ends | 9–90 fmol | 9–90 fmol |
T4 DNA ligase (1 U µl−1) | 1 µl | 1 U |
Nuclease-free water | Up to 20 µl |