Figure 6.
Enhanced tumor invasion by cells that overexpress lysophosphatidic acid (LPA) receptors. Female nude mice were injected intraperitoneally with lentivirus-transduced SKOV-3 cells (10 × 106 per mouse) expressing LacZ (n = 5), LPA1 (n = 4), LPA2 (n = 5), or LPA3 (n = 5) receptor. Seventeen days after injection, tumor-bearing mice were sacrificed and subjected to necropsy, and the organs were processed for hematoxylin and eosin staining. Pathological sections were examined for the presence of tumor invasion. Data are presented as the percentage of mice with invaded lesions (A) and the overall percentage of mice with tumor invasion in the peritoneal cavity (B). LPA receptor groups and the LacZ control were compared using the Student t test.