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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Biochemistry. 2008 Oct 15;47(45):11885–11893. doi: 10.1021/bi801499g

FIGURE 6.

FIGURE 6

X-Band EPR spectra of Mn(II)-substituted forms of EcMetAP-I: (A) [Mn_(H79A EcMetAP-I)], (B) [MnMn(H79A EcMetAP-I)], (C) [Mn_(WT EcMetAP-I)], and (D) [MnMn(WT EcMetAP-I)]. The high- and low-field wings of the spectrum of [Mn_(H79A EcMetAP-I)] are shown with a larger (5 times) amplitude, above and below, respectively, trace A. Traces E–H are of the geff ~ 2 regions of spectra A–D, respectively. Spectra were recorded at 8 K with a microwave power of 0.2 mW, a field modulation amplitude of 1.2 mT, a modulation frequency of 100 kHz, and a sweep rate of 10.2 mT s−1, and the samples were in 25 mM HEPES buffer and 150 mM KCl (pH 7).