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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Biochemistry. 2008 Oct 15;47(45):11885–11893. doi: 10.1021/bi801499g

Table 3.

ITC Data for the Mn(II)- and Co(II)-Loaded H79A EcMetAP-I Enzymes

na Ka1, Ka2 (M−1) Kd1, Kd2 (µM) ΔH°1, ΔH°2 (kcal/mol) TΔS°1, TΔS°2 (kcal/mol) ΔG°1, ΔG°2 (kcal/mol)
[CoCo(EcMetAP-I)] 1 6.36 × 105 1.6 15700 15708 −7.8
2 7.05 × 101 14200 1.1 × 106 1.1 × 106 −2.5
[MnMn(EcMetAP-I)] 2 3.31 × 105 3.0 2600 2607 −7.4
1 2.27 × 102 4400 33800 33803 −3.2
[CoCo(H79A)] 1 7.35 × 105 1.4 549 557 −7.9
2 6.41 × 102 1600 9770 9774 −3.8
[MnMn(H79A)] 1 1.90 × 105 5.3 −461 −454 −7.1
2 1.34 × 104 80 20900 20905 −5.5
a

All fits were performed using an n value of 3, providing two distinct binding events with individual n values of 1 and 2.