Table 1.
Percentage of dauer formation in wild-type and mutant strains
| Strain | Genotype | % dauer
formation
|
|
|---|---|---|---|
| 20°C* | 22.5°C† | ||
| N2 | Wild type |
0 | 0 |
| MQ470 | rop-1(pk93) | 0 | 0 |
| CB1370 | daf-2(e1370) | 4.4 ± 2.1‡ (6443; 8) | 40.0 ± 2.6‡ (1350; 3) |
| MQ844 | daf-2(e1370); rop-1(pk93) | 42.3 ± 6.4 (6010; 8) | 89.3 ± 3.4 (1826; 3) |
| CB1370+ | daf-16(RNAi); daf-2(e1370) | 0.8 ± 0.9 (820; 1) | ND |
| MQ844+ | daf-16(RNAi); daf-2(e1370); rop-1(pk93) | 0.7 ± 1.5 (350; 1) | ND |
| MQ845 | daf-2(e1370); rop-1(pk93); qmEx202 [rop-1(+); pRF4] | 6.4 ± 1.2 (2177; 4) | 59.7 ± 2.0 (740; 3) |
| DR1566 | daf-2(m579) | 4.2 ± 1.7‡ (3149; 3) | 60.9 ± 4.4‡ (2500; 3) |
| MQ846 | daf-2(m579); rop-1(pk93) | 74.7 ± 9.3 (2975; 3) | 99.1 ± 0.5 (1981; 3) |
| DR1564 | daf-2(m41) | 9.2 ± 1.2§ (4031; 4) | 99.5 ± 2.6§ (2019; 3) |
| MQ841 | daf-2(m41); rop-1(pk93) | 0.1 ± 0.1 (3836; 4) | 5.6 ± 1.9 (2713; 3) |
| DR1565 | daf-2(m596) | 2.2 ± 0.1§ (3179; 3) | 69.4 ± 3.9§ (2078; 3) |
| MQ847 | daf-2(m596); rop-1(pk93) | 0.2 ± 0.1 (2362; 3) | 15.4 ± 4.8 (1725; 3) |
| CB1372 | daf-7(e1372) | 16.1 ± 3.9 (5601; 7) | 89.7 ± 4.5¶ (1242; 2) |
| MQ804 | daf-7(e1372); rop-1(pk93) | 32.6 ± 3.8 (3982; 7) | 65.7 ± 4.3¶ (1001; 2) |
Experiments were performed as described by Gems et al. (21). The value corresponds to the percentage of dauer larvae formed from broods of 5–10 individual animals, depending on the trial (mean ± SEM across one–eight trials). The total number of animals scored is the first number given in parentheses. The number of trials is the second number given in parentheses.
*
Scored 96 h after hatching.
†
Scored 72 h after hatching.
‡
Many dauer-like animals with intermediate characteristics of L3 or L4 stage larvae.
§
Animals take 4 to 5 days to reach adulthood.
¶
This particular set of experiments was performed at 25°C.