Table 3.

Current Expansion Success of Cord Blood Mononuclear Cells

Cell Type(s)	Expansion Fold	Notes
CD34⁺ GM-CFU	12 and 20, respectively	Culture using a closed cell expansion chamber (3).
CD34⁺, CFU	27 and 22, respectively	7 days in serum-free culture (83).
CD34⁺		IL-11 and G-CSF stimulated, created 80-fold greater yield than BM (31,68,71).
CD34⁻/CD45⁻	10¹⁵	No loss of pluripotency (39).
Total MNC, CD34⁺, CFU-GM	435.5, 32.7, and 21.7, respectively	200 h culture in a rotating wall vessel bioreactor was used with serum containing media supplemented with low-dose recombinant human cytokines (43).
CD34⁺, CD45⁺, CFU	3, 3, and 2.6, respectively	Serum-free, cytokine-free 3D cytomatrix (20).
CD34⁺, CD34⁺/CD38⁻, CFU-C	250	Expanded on a monolayer of human BM MSC with cytokines and serum (81).
MSC	1000	In culture with serum for 3 weeks (82).