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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4899–4909. doi: 10.4049/jimmunol.0803242

Figure 5. Immunization With iFt Plus CTB Enhances Production of F. tularensis-Specific IgA.

Figure 5

BALB/c (A and B) and C57BL/6 (C) mice were divided into four and three groups respectively (5 mice/group) and immunized i.n. with 20μl of either PBS, 5μg of CTB in 20 μl PBS (only BALB/c), 2 × 107 iFt in 20 μl PBS, or 2 × 107 iFt plus 5μg of CTB in 20 μl PBS. Mice were immunized on day 0 and boosted on day 21 as in challenge studies in Figure 1. BAL fluid or sera was then collected at 35 days and tested for the presence of anti-F. tularensis IgA Ab by ELISA, as described in Materials and Methods. Ab data represent the mean of 4–5 mice + SD. The levels of IgA were significantly higher in the BAL fluid and sera of iFt plus CTB-immunized mice versus mice immunized with iFt alone (p<0.01 and p<0.05, respectively in BALB/c mice and p<0.01 in C57BL/6). These results are representative of two experiments.