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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: Chem Biol Interact. 2008 Oct 21;178(1-3):165–170. doi: 10.1016/j.cbi.2008.10.016

Fig. 1. Absorbance (A) and fluorescence (B) spectra of free and nucleotide-bound Kvβ2.

Fig. 1

Line 1 in both panels, optical density (A) and fluorescence (B) scans of Kvβ2 freshly purified from E.coli; line 2, scans after oxidation of protein-bound NADPH with 0.6 mM 4-nitrobenzaldehyde followed by 14–16 h dialysis. Fluorescence spectra in panel B were obtained using an excitation wavelength of 290 nm.