Table 3.
Enzyme activities (U/(mg total protein)): dehydrogenases
Enzyme | Substratea | Strain | |
LAD | L-ARABITOL | XYLITOL | |
0.77 ± 0.10 | 0.58 ± 0.07 | TMB3659 | |
ALXa, LAD, XDH | 0.94 ± 0.04 (0.45 ± 0.02) |
28.54 ± 4.91 (21.13 ± 5.57) |
TMB3664 |
XDH | 0.72 ± 0.01 (0.25 ± 0.00) |
7.20 ± 0.56 (7.88 ± 0.88) |
TMB3665 |
NAD+ was used as a cofactor for all reactions. a. Standard substrate concentration: 330 mM. Values between parentheses are referred to assays performed with substrate concentration of 100 mM.