Table 4.

Substrate consumption and product formation parameters during anaerobic batch fermentation of mixed sugars in defined mineral medium

		TMB3664 (D-xylose/L-arabinose pathway)		TMB3665 (D-xylose pathway)
	Medium	G-A-X	G-A	G-A-X
Overall process	Initial glucose g/l	20.7 ± 0.2	20.1 ± 0.9	20.1 ± 0.4
	Initial L-arabinose g/l	22.1 ± 0.2	23.1 ± 0.4	22.1 ± 0.1
	Initial xylose g/l	21.7 ± 0.4	-	20.8 ± 0.3
	Consumed arabinose g/l	3.9 ± 0.2	2.1 ± 0.1	3.1 ± 0.3
	Consumed xylose g/l	14.1 ± 0.2	-	13.7 ± 0.2
	Final ethanol titer+ g/l	15.3 ± 0.0	9.3 ± 0.4	14.3 ± 0.3
	Y ethanol, total sugars (g ethanol)/(g sugar)	0.23 ± 0.00	0.21 ± 0.01	0.22 ± 0.01
	Y ethanol, consumed sugars (g ethanol)/(g sugar)	0.40 ± 0.01	0.42 ± 0.01	0.39 ± 0.00
	Y xylitol, consumed xylose (g xylitol)/(g consumed D-xylose)	0.09 ± 0.01	-	0.10 ± 0.0
	Y xylitol, consumed C5 (g xylitol) (g consumed sugar)-1	0.07 ± 0.00	n.d.	0.08 ± 0.0
	Y arabitol, consumed arabinose (g L-arabitol)/(g consumed L-arabinose)	0.48 ± 0.01	0.39 ± 0.03	0.90 ± 0.07
Pentose Phasea	q arabinose (g L-arabinose)/(g cells)/h	0.021 ± 0.001	0.011 ± 0.001	0.013 ± 0.000
	q xylose (g D-xylose)/(g cells)/h	0.080 ± 0.001	-	0.077 ± 0.001
	q ethanol (g ethanol)/(g cells)/h	0.035 ± 0.001	0.003 ± 0.001	0.029 ± 0.001
	Biomass, produced from C5 g/l	0.37 ± 0.01	n.d.	0.25 ± 0.03
	Y biomass, produced from C5 (g biomass)/(g consumed sugar)	0.025 ± 0.001	-	0.019 ± 0.002
	Y ethanol, produced from C5 (g ethanol)/(g consumed sugar)	0.35 ± 0.01	0.35 ± 0.01	0.32 ± 0.01
	Y glycerol, produced from C5 (g glycerol)/(g sugar)	0.03 ± 0.01	0.06 ± 0.02	0.02 ± 0.00

Values are the calculated average of two biological replicates. Errors are given as mean deviations. G-A-X: medium containing glucose, L-arabinose and D-xylose; G-A: medium containing glucose and L-arabinose only. +: estimated based on the degree of reduction balance. a. values calculated after glucose depletion. q: specific productivity, Y: yield, C5: pentose sugar, n.d.: not detected.