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A PI3-kinase inhibitor prevents growth of cultured neonatal mouse metatarsal bones. Neonatal mouse metatarsals were incubated in DMEM containing 0.5% BSA with or without 20 μM LY294002 for up to 10 days. (A) Representative images of metatarsal bones after incubation with or without LY294002 for 10 days. (B) Relative change in metatarsal bone length after incubation with or without LY294002 for 4, 7 or 10 days compared with day 0 (mean ± s.d., n=3 experiments; *P<0.001 vs +LY294002).
