AktDN inhibits growth of cultured neonatal mouse metatarsal
bones. Neonatal mouse metatarsals were infected with Ad-EGFP and Ad-tTa, or
Ad-AktDN and Ad-tTa, and were incubated in DMEM containing 0.5% BSA
with or without Dox for up to 10 days. (A) Representative image of metatarsal
bone for EGFP expression at 10 days after infection with Ad-EGFP and Ad-tTa
without addition of Dox. In the presence of Dox no EGFP was detected. (B)
Immunoblot showing induction of AktDN in the absence of Dox, and
expression of endogenous Akt (lower band) and α-tubulin in tissue
lysates from metatarsals after 10 days of culture. (C) Representative images
of metatarsal bones after incubation with Ad-EGFP or Ad-AktDN with
or without Dox, or no adenovirus (Con) for 10 days. (D) Relative change in
metatarsal bone length after infection with Ad-AktDN for 4, 7, or
10 days with or without Dox treatment compared with day 0 (mean ± s.d.,
n=3 experiments; *P<0.001,
**P<0.01 vs +Dox).