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. 2009 Feb 10;122(5):716–726. doi: 10.1242/jcs.042770

Fig. 9.

Fig. 9.

AktDN inhibits growth of cultured neonatal mouse metatarsal bones. Neonatal mouse metatarsals were infected with Ad-EGFP and Ad-tTa, or Ad-AktDN and Ad-tTa, and were incubated in DMEM containing 0.5% BSA with or without Dox for up to 10 days. (A) Representative image of metatarsal bone for EGFP expression at 10 days after infection with Ad-EGFP and Ad-tTa without addition of Dox. In the presence of Dox no EGFP was detected. (B) Immunoblot showing induction of AktDN in the absence of Dox, and expression of endogenous Akt (lower band) and α-tubulin in tissue lysates from metatarsals after 10 days of culture. (C) Representative images of metatarsal bones after incubation with Ad-EGFP or Ad-AktDN with or without Dox, or no adenovirus (Con) for 10 days. (D) Relative change in metatarsal bone length after infection with Ad-AktDN for 4, 7, or 10 days with or without Dox treatment compared with day 0 (mean ± s.d., n=3 experiments; *P<0.001, **P<0.01 vs +Dox).