Figure 4.

RT PCR validation of expression profiles. Reverse transcription-polymerase chain reaction (RT PCR) was used to validate the nectary-enriched expression profiles of select genes identified through microarray analyses. The tissues examined included: 1) petal; 2) sepal; 3) rosette leaf; 4) stamen; 5) pistil; 6) root; 7) internode shoot; 8) silique; 9) mature median nectaries; 10) immature lateral nectaries; and, 11) mature lateral nectaries. Individual genes are described throughout the text and in Additional files 7, 9, and 10; UBQ5 (At3g62250) and GAPDH (At3g04120) were used as constitutively expressed controls.