(A) Sequence comparison of the two amino-terminal
Ig-like domains of CHIR-A, CHIR-B, mouse (mo) PIR-B (AF038149), moNKp46
(AJ223765), mogp49-B (2997305), rat (ra) PIR-B (AF16936), raNKp46
(AF082533), human (hu) LIR-1 (AF009220), huKIR2DL1 (AF022049), huFcαR
(U4774), huNKp46 (AJ001383), and bovine (bo) Fcγ2R (2136749). Gaps in
the alignment are indicated by dashes, and bold arrows represent
regions of β-stranded secondary structures (A–G) designated
according to the crystal structure of human KIR (42). Residues
identical in all of the aligned sequences are in red, yellow indicates
80% of the aligned sequences are identical, and blue indicates 60%
identity for the aligned sequences. (B) Comparison of
the CHIR-B, moPIR-B (AF038149), mogp49-B (2997305), raPIR-B (AF16936),
huLIR-1 (AF009220), huKIR (AF022049), and huLAIR (AF013249) cytoplasmic
tails, with gaps in this alignment indicated by dashes. Bold lines
indicate moPIR-B ITIMs demonstrated to have inhibitory function. Red
indicates residues that are identical in all of the aligned sequences,
yellow indicates identity in 60% of the aligned sequences, and blue
indicates 40% identity for the aligned sequences.