Table 4.

Nested ANCOVAs comparing Groups 1, 2 and 3 sacrificed at different times on the sixth day of wheel access

	Transform	N	Group	Line type	Mini muscle	Group×line type	Group×mini muscle	Estrous cycle	Age
Plasma glucose	None	186	F2,12=7.10	F1,6=3.27	F1,154=0.01	F2,12=1.1	F2,154=2.37	F4,154=085	F1,154=6.12
			P=0.0092	P=0.1203	P=0.9196	P=0.3625	P=0.0969	P=0.4972	P=0.0145
Glycogen gastrocnemius	^0.4	197	F2,12=0.13	F1,6=1.24	F1,165=92.39	F2,12=0.98	F2,165=3.04	F4,165=0.88	F1,165=0.01
			P=0.8761	P=0.3084	P<0.0001	P=0.4045	P=0.0503	P=0.4754	P=0.9140
Glycogen soleus	^0.6	196	F2,12=1.27	F1,6=6.53	F1,164=4.06	F2,12=4.63	F2,164=3.58	F4,164=3.20	F1,164=0.03
			P=0.3170	P=0.0431	P=0.0456	P=0.0324	P=0.0302	P=0.0146	P=0.8708
Glycogen liver	^0.5	197	F2,12=11.06	F1,6=0.68	F1,165=1.25	F2,12=3.28	F2,165=0.12	F4,165=4.00	F1,165=3.88
			P=0.0019	P=0.4422	P=0.2659	P=0.0729	P=0.8854	P=0.0040	P=0.0505

Data were transformed as indicated to improve normality of residuals. Bold font indicates 2-tailed P<0.05, unadjusted for multiple comparisons