Fig. 9.

Plectin is recruited to FAs in TrHBMECs in a kinesin-dependent manner. (A) TrHBMECs were plated in serum-free medium on PLL-coated coverslips overnight. Untreated cells (left panel) or cells incubated for 30 minutes in medium supplemented with 0.5 mM MnCl₂ (right panel) were processed for immunofluorescence microscopy using an antibody against plectin. (B) TrHBMECs electroporated with heavy chain dynein shRNA or heavy chain kinesin shRNA were plated in serum-free medium on PLL-coated coverslips overnight. Untreated cells (left panels) or cells incubated for 30 minutes in medium supplemented with 0.5 mM MnCl₂ (right panels) were processed for immunofluorescence microscopy using an antibody against plectin. Scale bars: 10 μm.