Fig. 1.

Effect of Acanthamoeba castellanii proteinase on human immunoglobulins. Immunoglobulins (10 µg) were incubated with the purified enzyme at 37℃ for 0.5 and 1 h, respectively. After incubation, samples were heated at 100℃ for 3 min and analyzed SDS-PAGE. (A) Secretory immunoglobulin A. (B) Immunoglobulin G. (C) Immunoglobulin M. Lane 1, no proteinase; Lanes 2-3; incubated with the proteinase (0.1 µg) at 37℃ for 0.5 and 1 h,