Profile of ESP of Toxoplasma gondii at various temperatures. Western blot was reacted with a reference serum from a mouse infected with Me49 strain. RH, tachyzoites; 4, 25, 37, and 42, temperature to incubate tachyzoites for 1 hr. Five ESP (a to e) were detected by mAbs (a, Tg386; b, Tg485; c, Tg786; d, Tg378; and e, Tg556) among 15 bands released at 37℃ indicated by dots.